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Approximate glutathione redox states can be estimated by simply comparing GS-bimane peak yields in aliquots from the same extract pre-treated or not with reductants like DTT, though the alkaline pH required for reaction with MBB probably causes some artefactual oxidation of extracted GSH if DTT is not present during the derivatization

Plasma asymmetric dimethylarginine (ADMA) concentration is independently associated with carotid intima-media thickness and plasma soluble vascular cell adhesin molecule-1 (sVCAM-1) concentration in patients with mild-to-moderate renal failure

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Blum, R

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These findings all indicate that ferroptosis promotes the development of DCM through abnormal iron metabolism, lipid peroxidation, ROS production, and enhanced cardiomyocyte inflammation (Figure 1)