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On day four, resazurin was added to a final concentration of 30 mol L 1 and fluorescence determined the next day

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Rocha CR, Garcia CC, Vieira DB, Quinet A, de Andrade-Lima LC, Munford V, et al

Despite their clinical heterogeneity, these disorders share convergent pathological drivers, including oxidative stress, chronic inflammation, hormonal dysregulation, metabolic dysfunction, aberrant extracellular matrix (ECM) remodeling, and impaired apoptosis (Kumar and Ramanarayanan, 2025)

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However, the hydrophobic pocket formed by the three aromatic residues has been suggested to shield the catalytic nucleophile from bulk solvent and activating hydrolysis