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PreissHandler and de novo do not occur within the mitochondria, based on the nuclear or cytosolic localization of QPRT, NAPRT, and NADS 48
doi: 10.1016/j.cell.2010.10.002
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Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayers hematoxylin nuclear counter stain as per manufacturers instructions
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