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Cell supernatants were harvested for quantification of IL-1 by ELISA following the manufacturers directions (Sigma-Aldrich, USA)
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In addition, it has been demonstrated to attenuate oxalate-induced AKI [74]
For bioconjugation of Fc, human IgG1 Fc has been widely used, although it has disadvantages such as antibody-dependent cell cytotoxicity (ADCC) and complementary-dependent cytotoxicity (CDC) 12
