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Comparative analysis of inducible expression systems in transient transfection studies

Sirtori CR, Calabresi L, Franceschini G, Baldassarre D, Amato M, Johansson J, Salvetti M, Monteduro C, Zulli R, Muiesan ML, Agabiti-Rosei E

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Diazepam-induced loss of inhibitory synapses mediated by PLC/Ca 2+ /calcineurin signalling downstream of GABAA receptors

Furthermore, intracellular Flag-ATG2A precipitated using anti-Flag beads displayed positive staining for 17-octadecynoic acid (17-ODYA), a bioorthogonal click chemistry probe for in situ labeling of palmitoyl protein (Martin et al, 2011

Buffer A (95% H 2 O, 5% methanol, 5 mM ammonium acetatepH adjusted to 5.0 with acetic acid) and buffer B (95% isopropanol, 5% methanol) were used