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Exp Neurobiol 22:1117 Markesbery WR (1997) Oxidative stress hypothesis in Alzheimer's disease

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These additives include: Phenylmethylsulfonyl fluoride (PMSF, dissolved in very small amount of propanol just before extraction) to inhibit protease Flavin compounds (FAD) Dithiothreitol (DTT), to preserve the sulfhydryl group EDTA, as chemical chelator especially with phosphate buffer Sodium fluoride to inhibit phosphatase Polyphenylpyrovate (PVP) 25 gm/k gm fresh weight of sample, which is an insoluble compound and binds to phenolic compound present in the sample, the obtained compound discarded by centrifugation 30% glycerol, which may help to stabilize some highly labile proteins Antibiotic such as Hibitane, usually used in protein extracted from rhizomes or underground plant parts in general Filter the homogenate with nylon mush, then centrifuge at 10,000 g for 10 min using cooling centrifuge

doi: 10.3390/ijms19082287 56 Otulak-KozieK.KozieE.HorvthE.CsiszrJ

Patients with renal disease who undergo hemodialysis are at risk for secondary carnitine deficiency because hemodialysis removes carnitine from the blood (see End-stage renal disease) (38)