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(2) Risks to subjects are reasonable in relation to anticipated benefits, if any, to subjects, and the importance of the knowledge that may be expected to result

Because vitamin B 12 in supplements is not bound to protein, and because intrinsic factor (IF) is still available, the absorption of supplemental vitamin B 12 is not reduced as it is in pernicious anemia

This is upstream of histamine release, meaning it prevents the problem rather than managing its consequences

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The tryptophan residue at the N-terminus gives it a characteristic UV absorbance profile used for laboratory identification
Vitamin B12 is water-soluble, and the body excretes excess amounts through urine rather than storing supraphysiological levels
