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Carnosine, abundant in the skeletal muscle, has a role as a pH buffer and protects the muscle against acidosis created during anaerobic energy production, the end products of which are lactate anions and H + [78]

doi: 10.1089/thy.2007.0069 30 PlioplysAPlioplysS

In the dry state it is reasonably stable in air, but in solution it rapidly oxidizes

Preparation of the muscle specimens and frozen section To prepare frozen muscle sections, we first anesthetized adult fish with a 0.025% buffered MS-222 solution (Sigma-Aldrich, US), and then removed a segment of muscle tissue located between the anal and caudal fins 35

A follow-up phase 2 study reported minimal adverse effects associated with this therapy (Stein et al., 2019), and there are several clinical trials further investigating this protein (NCT05234775, NCT04797247, NCT05004675, NCT03545438, NCT04034485, NCT04790513, NCT04806893, NCT04797104, NCT04798430, NCT03549260)

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