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Several considerations are typically accounted for, besides accurate mass and RT, which includes the elimination of background signals, deisotoping, identification of adduct peaks in MS spectra, and MS/MS fragmentation patterns compared to databases or internal standards and the matching degree estimated (see Figure 2)

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Competing interests: The authors have no conflicts of interest to disclose

Increases in the levels of intracellular aspartate via SLC1A3 contribute to nucleotide synthesis and maintain the electron transport chain and TCA cycle 114

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