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The binding of CLOCK and BMAL1 to these E-boxes initiates the transcription of key clock genes, specifically the Period (PER) and Cryptochrome (CRY) genes, which encode proteins crucial for maintaining the circadian rhythm [30, 32, 34]

Lancet (2020) 395:184554

After 72 h, cells were harvested and subjected to Western blotting assays

The draw volume then depends on the intended dose and the concentration

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Plant roots show a reduction in the concentration of essential nutrients (Ca, K, and F) and a decrease in root length and biomass in the presence of toxic levels of 480 M of Cu [53], suggesting that morphological changes in roots and shoots induced by Cu are linked to nutritional disorders in higher plants exposed in the medium and long term to toxic levels of Cu