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Mistake 2: Using GST When You Need Monomeric Protein The fix: If your downstream assay is sensitive to oligomeric state (SEC-MALS, AUC, SPR), avoid GST or plan to cleave and separate

During the process of making energy, the mitochondria produce "exhaust" in the form of free radicals

Footnotes Conflict of interest: The authors declare no potential conflicts of interest

Oral supplements can be recommended for some dogs or an injection of cyanocobalamin (usually 1,000 mcg/mL) may be recommended for dogs with severe deficiencies or poor intestinal absorption

More serious but less common side effects require immediate medical attention

Ruiz-Hernndez IM, Nouri M-Z, Kozuch M, Denslow ND, Daz-Gamboa RE, Rodrguez-Canul R, et al