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At least 70 enzymes have been identified as requiring CoA or ACP derivatives for their function including the same critical thiamine-dependent enzymes described above, the pyruvate dehydrogenase complex (PDHc), 2-oxoglutarate (-ketoglutarate) dehydrogenase complex (OGDH), and branched-chain -ketoacid dehydrogenase complex (BCKD)

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Although it has been shown that GST has a significant role in detoxification of aflatoxin and there is a possibility that this enzyme catalyses the conjugation of GSH to AFB 1 - epoxide to excrete its derivatives from the body, in 1988 and for the first time Saxena et al

GST-Gid7 was purified by glutathione affinity chromatography followed by incubation overnight at 4 C with TEV protease at a concentration of 0.05 mg/ml to remove the GST tag

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