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Cell pellets were collected and resuspended in lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl, 5 mM -mercaptoethanol, 10% glycerol, and 1X Halt protease inhibitor (Thermo Fisher), and frozen overnight at 80 C
[DOI] [PMC free article] [PubMed] [Google Scholar] 7.Miller A.J., Arnold A.C
Never share a multi-dose vial between different patients
Janet Beres, PA-C Table of Contents: What are the symptoms of B12 deficiency
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