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Galangin-triggered cell apoptosis was characterized by DNA breaks, caspase-3/9 activation, PARP cleavage, and coordinated activation of the expression of MAPK kinases (ERK and JNK) in human gastric cancer SNU-484 (Sabbah et al., 2020)

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Cancers 14 , 5597 (2022)

Immunofluorescence (IF) assay For cellular immunofluorescence, the cells were placed on a chamber slide and fixed with ice formaldehyde for 5 min

They found that, compared with healthy controls, FMS sufferers had lower levels of CoQ10 and ATP, increased oxidative stress, inflammation and mitochondrial dysfunction, which were to some extent explained by changes in gene expression

After infectious or sterile stimuli, SLC15A4 in DCs is recruited to phagosomes, SLC15A4deficient DCs infected with Salmonella typhimurium show reduced caspase-1 cleavage and IL-1 production, this correlates with peripheral NLRC4 inflammasome assembly and increased autophagy