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PKH67-labeled sEVs were incubated with VSMCs for 12 h, fixed with 4% paraformaldehyde for 10 min at room temperature, and washed twice with PBS

In a mixed model ANCOVA, the pretreatment TEE depended on BW, but also on experiment (adjusted (coefficient of determination) R 2 = 0.471, 68% of attributable variation due to BW and 32% to experiment)

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