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After induction, these cultures were incubated for 4 h at 25C to maximize protein solubility

PMID: 24324897

Data were recorded as a percentage of the number of times the mouse completed a triad or loop through all three arms throughout the series of arm locations during the trial time divided by the number of entries minus two

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Additionally, immunostaining was done on hepatic sections for the SIRT -1 marker

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