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The priming phase is triggered by stimuli such as TLRs or TNF-, which induce transcription of NLRP3 and pro-IL-1 via the nuclear factor kappa-B (NF-B) pathway
Neurology 63: 139144, 2004
The cDNA was diluted and used as a template for real-time PCR using the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) with Thunderbird Next SYBR (Toyobo, QPX-201), following the manufacturers guidelines

Mitochondrial Dysfunction: A Common Denominator in Neurodevelopmental Disorders? Developmental Neuroscience 43: 222229

Mechanisms include reduction of excitotoxicity and oxidative stress, enhanced neurotrophic factor expression supporting neuronal survival, angiogenesis improving cerebral blood flow, and modulation of dopamine and serotonin systems
