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Transfected HeLa cells were starved for 1 h in MEM supplemented with 10 mM HEPES, pH 7.4, and subsequently radiolabeled for 3 h with 70 Ci/ml of [9,10- 3 H]palmitate (American Radiolabeled Chemicals, Inc., ART-0129-25)

The samples were incubated at room temperature

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