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Lysates were denatured by boiling in Nupage loading Buffer (Cat#NP0007) and Sample Reducing agent (Cat#NP0004) (10 min, 90 C) and resolved in Nupage 412% gels (Cat#NP0321BOX) using MOPS SDS running buffer (Cat#NP0001)
Molecular insight into 2-phosphoglycolate activation of the phosphatase activity of bisphosphoglycerate mutase
Mohamed et al., 2004)
Austin, C., et al
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