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pylori s general protein glycosylation system in modulating immune response is less clear

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The negative effects are mostly due to the injection process, which leads to a compromised shell and membrane of the egg, allowing for pathogenic bacteria and environmental factors affecting the egg and embryo

2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)