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The rate of decrease of absorbance at 340 nm is directly proportional to LDH or SDH activity in the sample, where one unit of LDH or SDH activity is defined as 0.1 mol of substrate transformed/minute at pH 7.6 at 25C.The SDH enzymatic activity was determined as the amount of fructose reacted per unit of time, and it was measured by recording the absorbance decrease due to NADH oxidation

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One of critical pathogenic events contributing to EtOH-induced lipotoxicity is the inhibitory action of EtOH on the AMPK pathway 21,22

Injectable use in humans represents off-label experimental application with limited long-term human safety data specifically for injectable formulations

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