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For most people, taking 2 grams or less per day is relatively safe and does not cause serious side effects

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Associations of current diet with plasma and urine TMAO in the KarMeN study: direct and indirect contributions

Trends Endocrinol

In fact, FMO1 is also participated in the oxidization of TMA to TMAO, however, its specific activity is only 1/10 that of FMO3 ( Numerous studies indicate that inhibition of FMOs, especially FMO3, is an effective target for reducing TMAO concentrations (Zhu et al., 2018

In general, consuming it 30 to 60 minutes before exercise can maximize the benefits related to performance and endurance, while taking it after exercise can support muscle recovery