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Radioactivity was measured with the TopCount scintillation counter (Perkin Elmer)

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The cells were again suspended in bacterial lysis solution (20% glucose, 10% glycerol, 2 mM MgCl2, 50 mM TrisCl at pH 8.0)

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Moreover, alterations in NuAc function are implicated in psychopathological conditions, that involve motivational deficits, such as depression 2225