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TNBC cell lines HCC1806 and HCC1143, luminal A breast cancer cell lines T-47D, MCF-7 and ZR-75-1 and the HER2-positive breast cancer cell lines BT-474, SK-BR-3 and ZR-75-30 were maintained in RPMI1640 medium (Gibco) supplemented with 10% FBS, 25 mM HEPES, 1 mM Sodium Pyruvate and 100 U/ml Penicillin/Streptomycin

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Gene expression by QPCR in the fat-5, fat-6, and fat-7 mutants relative to wild type reveals an increase in 9 desaturase gene expression in the fat-6 and fat-7 mutants, relative to wild type

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