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Analysis of single-cell RNA sequencing Single-cell RNA sequencing (scRNA-seq) data (HRA000425) were downloaded from Genome Sequence Archive (SRA)-Human databases in China National Center for Bioinformation 16 , converted into FASTQ format using fasterq-dump software (sratoolkit 3.0.2, NCBI, NIH, Bethesda, MD, USA), converted into a matrix data file using Cell Ranger software version 3.0.2 (10xGenomics, Pleasanton, CA, USA), converted into Seurat objects using Seurat 5.0.1 software (Satija lab, Lower Manhattan, New York, NY, USA), and plotted for t -distributed stochastic neighbor embedding ( t -SNE) visualization

The nickel filters were used to pass the single beam of X-rays

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Ethics declarations Ethics approval The Institutional Animal Ethics Committee permitted the study protocol (approval no

Female C57BL/6J mice (10 weeks old) were fasted for 4 h and infected orally with 1 10 10 c.f.u.s in 200 l PBS

Quality determines outcomes more than any other factor