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The first such letter to the Chelsea Hospital we have seen

Cells were centrifuged at 900 rpm for 5 min and washed with HBSS

Especially if you a salt from a weak base with a counterion

Li et al., 2017b

Moreover, cancer cells expressing mutant p53 exhibit increased sensitivity to mTOR inhibition compared to cancer cells with WT p53 [81]

J Biol Chem 283:3222932235
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