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The gradient was as follows: 0 min, 15% A

[DOI] [PMC free article] [PubMed] [Google Scholar] 51.Lpez-Mirabal H.R., Winther J.R

quantified 4,405 proteins in murine hepatic organoids and observed differentiation-dependent upregulation of CYP450 enzymes, hepatic transporters, and other metabolic proteins, which parallels our observations in human iHLOs 14

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These changes are accompanied by significant deficiencies in antioxidant enzymes compared to healthy subjects

While melt shift analysis helps identify proteins with biophysical changes, TPCA is a method that more specifically monitors binary protein interactions and protein complex dynamics (75)