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Description
The his-tag on your protein of interest, typically 6 to 10 histidine residues in a row, will bind more strongly to nickel , however
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As a rule of thumb, start low, use consistently, and only escalate if a lower concentration is well tolerated and you have a specific reason to

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Notably, addition of Lip-1 abrogated the GSSG-mediated cellular death (Figure 3K)
