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Total glutathione was quantified using 5,5-dithio-bis (2-nitrobenzoic acid) (DTNB) in the presence of glutathione reductase, as described by Ascorbate (AsA), dehydroascorbate (DHA), and total ascorbate were measured following 3 PO 4 , --bipyridyl, and FeCl 3 , incubating at 37C for 1 hour, and measuring absorbance at 525 nm

10.1016/0022-1910(67)90090-x 58 HuangH

[DOI] [PubMed] [Google Scholar] 246.Latzman JM, Kong L, Liu C, Samadani U

A novel antimicrobial peptide found in Pelophylax nigromaculatus

T4 ligase was used to ligate the fragments into the pET-28a and pGEX-4T-1 vectors

Mol Plant Pathol 18:4554 Zotti M, Dos Santos EA, Cagliari D, Christiaens O, Taning CNT, Smagghe G (2018) RNA interference technology in crop protection against arthropod pests, pathogens and nematodes