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D-alanyl ester depletion of teichoic acids in Lactobacillus reuteri 100-23 results in impaired colonization of the mouse gastrointestinal tract

To block protein thiols, methanol-fixed ECs were incubated with 200 mM iodoacetamide in 100 mM Tris (pH 8.3) and 5 mM EDTA at 37 C for 1 h

These isomers can be easily separated by normal-phase HPLC, the erythro form being the first eluting, and then saponified by mild alkali treatment

[DOI] [PMC free article] [PubMed] [Google Scholar] 180.Hasanuzzaman M., Bhuyan M.H.M.B., Zulfiqar F., Raza A., Mohsin S.M., Mahmud J.A., Fujita M., Fotopoulos V

A second aminoacid residue of mechanistic importance is Arg15, which is widely conserved among the known alpha class GSTs ( a value of the thiol group, and mutation of Arg15 to Ala or His decreases the steroid isomerase activity by two orders of magnitude, twice the effect of the Tyr9Phe mutation ( The mechanistic studies of human GST A1-1 were extended to human GST A3-3 following the discovery of the latter enzyme (Johansson and Mannervik, 2001)