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[PubMed] [Google Scholar] 203.Obochi G, Malu S, Obi-Abang M, Alozie Y, Iyam M

Contains 20mL of GlutaStat NS for IntraNasal Application

A dilution series of 05 mM GSH, together with 2.5 mM CDNB (from a 62.5 mM stock solution in ethanol), in GST assay buffer (25 L/well) was mixed with an equal volume of the protein solution (1 M GST, 2.5 mM CDNB in GST assay buffer) in a 96-well flat bottom microtest plate (Sarstedt, Nmbrecht, Germany) at 30 C and the absorbance at 352 nm was measured every 20 s for 10 min in a Synergy 2 microtiter plate reader (BioTek, Winooski, VT) using Gen5 version 1.9 software (BioTek)

This reaction is reversible under certain conditions, although it generally proceeds in the direction of SAH hydrolysis to prevent SAH accumulation and its inhibitory effects on methyltransferases

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Do not attempt to filter or clarify the solution