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doi: 10.5402/2012/137289
P.ColeR.DankertJ.FroschM.van PuttenJ
Ann Surg 246 (3): 375-81
Having immobilized the GSTs on the Strep -Tactin columns, the washed proteins were selectively desorbed using desthiobiotin and then solvent-extracted with methanol
For erinacine C production, the optimal medium was found to include 5 g/l oatmeal, 1.5 g/l calcium carbonate, and 0.5 g/l Edamin K at pH 7.5, which can generate concentrations up to 2.73 g/l after six days of cultivation [33]
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