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doi: 10.3390/ijms22126574 57 Delgado-RocheL.MestaF
Isolated ILC2s were cultured at 37 C (2 10 4 /ml) for 72 h in cRPMi supplemented with rhIL-2 (20 ng/ml
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and 150 rpm in Sf-900 II serum-free medium (SFM) (Life Technologies, Inc., Gaithersburg, Md.) in dry-air incubators and were subcultured from a starting density of 0.2 10 6 cells/ml to a final density of 1.0 10 7 cells/ml as suspension cultures up to passage 70
The GLUD2 gene is thought to have arisen as a result of a retrotranspostitional event to the X chromosome
And the Wistars of PCOS group were divided into three groups: PCOS + EA group ( n = 11), PCOS + exercise group ( n = 13) and PCOS group ( n = 12)
