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sclerotiorum (isolate #3) were produced by bisecting a sclerotium, placing it face down on PDA, and incubating at 25C for 4 days under continuous darkness (24 h) to encourage mycelial growth

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Breakthroughs in cell densities, doubling times, and bioreactor efficiency have demonstrated the potential for cost reductions, with one optimized system lowering costs from $437,000 to $1.95/kg (Risner et al

2006 doi: 10.1073/pnas.0511304103

only a dramatic GSH:GSSG ratio decline (i.e., from 100:1 to 1:1) would result in a 50% conversion of PSH into PSSG

We, therefore, analyzed this by flow cytometry and qPCR