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No evidence of cancer risk
PCR cycling conditions were as follows: 95 C for 3 minutes, followed by 30 cycles of 94 C for 30 seconds, 55 C for 45 seconds, 72 C for 1 minute, with a final extension at 72 C for 7 minutes and holding at 4 C
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Possible reasons could be that (1) DAB is preferred by LaccID over other aromatic substrates, and (2) DAB labeling was performed in sodium cacodylate buffer, which does not contain laccase inhibitors such as halides and thiols
doi: 10.1016/j.redox.2020.101480 [DOI] [PMC free article] [PubMed] [Google Scholar] [90].Finaud J, Lac G, Filaire E
