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Description
The following formula was used to calculate percentage cytotoxicity [22]: Measurements of malondialdehyde and iron levels Intracellular malondialdehyde (MDA) content was measured using a fluorometric lipid peroxidation assay kit (Abcam, 118970) following the manufacturers instructions

Borden, M

doi: 10.1038/nrurol.2012.198

Imagine yourself

Nature 472, 476480

Next, 900 l serum extract was dried in a vacuum evaporator (Thermo), reconstituted in 90 l of 50% acetonitrile (A955, Fisher Scientific) and transferred to glass vials for LCMS analysis