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Cells were fixed with 4% PFA for 10 min at room temperature (RT) and permeabilized with 0.15% Triton X-100 in PBS for 15 min

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The homogenized samples were centrifuged at 15,000 rpm at 4 C for 15 min and separated for measurement 35

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PE-22-28 PE-22-28 is a seven-amino-acid fragment derived from BDNF itself

Nutrients 12(4):1067
