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Reconstitution Protocol Recommended Solvent: Bacteriostatic Water (0.9% Benzyl Alcohol) 1 Allow to Reach Room Temperature Remove vial from cold storage

The molecule contains free thiol (-SH) groups that physically break the disulfide bonds cross-linking the glycoproteins in mucus

Slides were incubated for 1 h at room temperature in dark with secondary antibodies raised in different species and conjugated to different fluorochromes (FITC conjugated against rabbit [Cat #F9887] and DyLight 650-conjugated against mouse [Cat # ab97018])

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5fi and Extended Data Fig