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Finally, blots were washed during 5 minutes three times with TTBS and detection was carried out using ECL Western blotting detection reagent (Amersham, GE HealthcareBio-Sciences AB, Uppsala, Sweden)

Regarding the receptor nuclear localization, one of the genes reported to be overexpressed after IGF1R interaction with LEF1 in the nucleus is Axin2 (50), an expression which we also found to be increased in U87WT clones in basal conditions ( Supplementary Table S1 )

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After a single 50 mg oral dose, maximum plasma concentrations of CMI occur within 2 to 6 hours (mean, 4.7 hr) and range from 56 ng/mL to 154 ng/mL (mean, 92 ng/mL)

Early animal studies published in the *Journal of Endocrinology* showed that AOD-9604 could stimulate lipolysis (fat breakdown) and inhibit lipogenesis (fat creation) in obese Zucker rats