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Embracing a systems biology approach, rather than focusing solely on individual genes or metabolic pathways independently, holds immense potential [225]

10.1177/1756284820974917 [DOI] [PMC free article] [PubMed] [Google Scholar] 129.Vivarelli F, Canistro D, Cirillo S, Papi A, Spisni E, Vornoli A, et al

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P.ReddyV

During AVS, blood samples were collected from the inferior vena cava before and 1 h after adrenocorticotropic hormone (ACTH) stimulation to measure the PACs

After fixing with paraformaldehyde (4%) for 15 minutes, the cells were stained with either BODIPY or Nile Red dye for 15 minutes, followed by DAPI staining
