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For endogenous immunoprecipitation, the cells were lysed, the lysates were incubated with anti-WDR24 antibody (rabbit IgG used as control) and protein A/G magnetic beads (MCE) overnight at 4 C, washed five times with 0.1% Tween-20 and boiled with SDS loading buffer for 10 min 68
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It has been found to be beneficial in reducing the appearance of hyperpigmentation, a common skin concern characterized by dark patches on the skin
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Additionally, when estrogen activity decreased, learning capabilities were diminished (3)
(2016) 113:E547280
