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As we explained in the FY 2016 IPPS/LTCH PPS final rule (80 FR 49490 through 49491) and the FY 2017 IPPS/LTCH PPS final rule (81 FR 56914), section 1886(d)(3)(E) of the Act requires the Secretary to adjust the proportion of hospitals' costs attributable to wages and wage-related costs for area differences reflecting the relative hospital wage level in the geographic areas of the hospital compared to the national average hospital wage level

Quantitative real-time polymerasevchain reactions analysis (qPCR) Total RNA was extracted from granulosa cells using TRIzol reagent (1000 l TRIzol per 10^7 cells)

Agric Tcn Mx 25:107114 Plaut Z, Butow BJ, Blumenthal CS, Wrigley CW (2004) Transport of dry matter into developing wheat kernels and its contribution to grain yield under post anthesis water deficit and elevated temperature

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