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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor

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Pharmacological inhibition of the LIF/LIFR autocrine loop reveals vulnerability of ovarian cancer cells to ferroptosis

A recent study examined the mutant NQO1 * 3 protein using biochemical and structural analysis (X-ray crystallography, 2D-NMR) and concluded that the NQO1 * 3 variant is very similar to the wildtype NQO1 * 1 protein and that there were no obvious variations to explain the observed decreased activity (Lienhart et al., Alternatively spliced forms of NQO1 Initial characterization of the NQO1 gene (6 exons and 5 introns) by Jaiswal et al