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Samples were then incubated with primary antibodies in blocking buffer overnight at 4 C, washed three times with PBTA, then incubated with secondary antibody in blocking buffer for 1 h at room temperature

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Guo C, Che X, Briese T, Ranjan A, Allicock O, Yates RA, et al

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It's a gaseous hormone that helps blood vessels flex and expand, promoting proper blood flow and nutrient delivery

My skin tone leans more towards olive, but I did dust a bit of bronzer on my face here before applying the colors