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On the other hand, a major challenge of cell-free approaches is that metal selectivities of the metallosensors tend to be reinforced by the cellular milieu, i.e., the buffering molecules in cells (Section 2.2

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Table 6: Treatment of folate deficiency (adapted from Means 2021) 27 TOP Ongoing Care Ongoing Management Vitamin B 12 Duration of therapy Once a diagnosis of B 12 deficiency due to poor absorption of B 12 has been made, therapy should be maintained lifelong

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