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doi: 10.3390/molecules29163941 255

Methods Cell culture and growth conditions All cell lines were cultured in either RPMI (Gibco, 11875119) or DMEM media (Gibco, 12430063) supplemented with 10% fetal bovine serum (Gibco, 12483020) and 1% penicillin-streptomycin (Gibco, 15140-122) and passaged by washing with Dulbeccos phosphate buffered saline (PBS) (Gibco, 14190250), and incubating with trypsin-EDTA (0.25%) (Gibco, 25200114)

B.DengG

First, research has shown that IF can lead to nutritional deficiencies due to reduced intake during fasting periods [53, 119]

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