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Two subsequent PCRs, PCR2 and 3, generate megaprimer that contains the complete desired gene, tags, Kozak sequence, and flanking regions (blue) that anneal to the pCS2+8 plasmid downstream of the SP6 promoter and upstream of the SV40 poly-A tail
Our clinical trial was registered in Iranian Registry of Clinical Trial with code number of (IRCT 2014010716120 N1)

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9,62,63,64 For instance, copper participates in myelination and interacts with synaptic proteins and neurotransmitter receptors, highlighting its significant function at synapses
