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Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 100 g/mL penicillin/streptomycin/gentamicin (Invitrogen, Gibco) and in the condition of 95% air and 5% CO 2 humidified atmosphere at 37 C

Moderate concentrations of H 2 O 2 promote apoptosis, while high concentrations may lead to necrosis [81]

Gegg ME, Cooper JM, Schapira AH, Taanman JW (2009) Silencing of PINK1 expression affects mitochondrial DNA and oxidative phosphorylation in dopaminergic cells