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Thereafter, cells were permeabilized and stained in Intracellular Staining Permeabilization Wash Buffer (Biolegend, #421002) with added antibodies against intracellular antigens

Plump A, Smith J, Hayek T, Aalto-Setala K, Walsh A, Verstuyft J, Rubin E, Breslow J

This may be because sarcomas often form during growth spurts

Mod Pathol 30(11):1538 Veenhuizen KC, De Wit PE, Mooi WJ, Scheffer E, Verbeek AL, Ruiter DJ (1997) Quality assessment by expert opinion in melanoma pathology: experience of the pathology panel of the Dutch melanoma working party

Nutrition and Metabolism 2010;7(30):1-14

The molecule is structurally modified with an N-terminal trans-3-hexenoyl group, which dramatically reduces its susceptibility to dipeptidyl peptidase IV (DPP-IV) degradation, the enzyme that breaks down natural GHRH