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A dramatically increased number of autophagosomes (shown by GFP-LC3 puncta) was observed (Fig

Particularly, with long-term use, whether antioxidants are tolerable or whether there are possible side effects, like potential effects on the microbial community, need to be further examined

Nature 353, 273276

After cells were treated with NAC or Mito 10 -NAC for 24 h, eight baseline OCR measurements were taken before injection of oligomycin (1 mg/mL) to inhibit ATP synthase, dinitrophenol (50 M) to uncouple the mitochondria and yield maximal OCR, and inhibitors of complexes I and III (1 M rotenone and antimycin) to inhibit mitochondrial respiration

Plateau after initial improvement Some researchers experience initial improvements that eventually plateau

The dehydrated samples were then embedded in epoxy resin and polymerized at 60 C for 2 h